DNA-directed DNA Polymerase Activity in Oncogenic RNA Viruses

Characterization of the products of DNA-directed DNA polymerases in oncogenic RNA viruses.

THIS quotation is taken from t ho beginning of an uns igned article l headed "Central dogma reversed It. recount ing t he very important work of Dr H oward Temin' and others' showing that a n R NA t umour v irus can use viral R NA as a template for DNA synthesis . This is not t he first t ime that t he idea. of t ho cent ral . dogma has been misunderstood , in one way or another. In t his artic...

Normal human placentas contain RNA-directed DNA polymerase activity like that in viruses.

Extracts from over 100 normal human placentas have been examined for RNA-directed DNA polymerase (DNA nucleotidyltransferase, EC 2.7.7.7) activity. More than 80% of these placentas contained this enzyme activity, which banded at a density of 1.15-1.17 g/ml in sucrose. After heat treatment, this enzyme activity was shifted in density to 1.22-1.24 g/ml. The enzymatic activity was greater with (rC...

RNA-directed DNA-polymerase activity in Greene hamster melanoma.

The present investigation is concerned with the search for RNA-directed DNApolymerase activity in human and hamster melanoma cells in both in vivo tumors and tumor cells grown in tissue culture. The assays and techniques employed are based upon model studies carried out in this laboratory with the enzyme from avian myeloblastoses virus, and feline sarcoma, and lymphoma virus. In recent years, s...

DNA polymerase activity associated with RNA tumor viruses.

C-type RNA viruses, originating in mouse, cat, hamster, and viper, catalyze the synthesis of DNA from its constituent deoxyribonucleoside triphosphates. Both the rate and extent of the reaction were significantly enhanced by brief treatment of the intact virus with ether. A proportion of the newly synthesized DNA was associated with virions when intact virus was used as template; this was not t...

RNA-dependent DNA polymerase in virions of RNA tumour viruses.